Some research here and there

Riva

Lister
Background

In 2001, Ralph Baric, among others applied for and got a U.S. Patent 6,593,111 (https://patents.google.com/patent/US6593111B2/en?oq=6,593,111) . This patent was for “A method of directionally assembling a recombinant viral genome, comprising: obtaining a set of subclones of the viral genome, wherein each termini of each subclone is a restriction enzyme recognition site; and then ligating the subclones to assemble a recombinant viral genome; (5) wherein the viral genome is a coronavirus genome.”

The summary of the invention states “The present inventors have successfully assembled a full-length infectious clone of transmissible gastroenteritis virus (TGE). Using a novel approach, six adjoining cDNA subclones that span the entire TGE genome were isolated. Each clone was engineered with unique flanking interconnecting junctions which dictate a precise, systematic assembly with only the correct adjacent cDNA subclones, resulting in an intact TGE cDNA construct of about approximately 28.5 Kb in length. Transcripts derived from the full-length TGE construct were found to be infectious, and progeny virions were serially passaged in permissive host cells. Viral antigen and subgenomic mRNA synthesis were evident during infection and throughout passage. “

This patent was for a method of assembly of infectious clones of transmissible gastroenteritis virus (TGE) whereby they used Coronavirus genomes as example. From my understanding, they took wild viral genomes then reassembled them to be more infectious but with the same viral function as the natural genome. i.e. making a viral coronavirus that was not previously infectious to be more infectious without altering its viral function.

In 2002–2004 there was the outbreak of severe acute respiratory syndrome (SARS), caused by severe acute respiratory syndrome coronavirus (SARS-CoV or SARS-CoV-1), infected over 8,000 people from 29 different countries and territories, and resulted in at least 774 deaths worldwide.

Between 2003 and 2007, CDC funded a grant which resulted in the isolation of a human coronavirus (SARS-CoV). They also patented the SARS-CoV and the method of detection in 2007 (https://patents.google.com/patent/US7220852B1/en?oq=7,220,852).

One of the interesting things from this patent application was that it said “Coronavirus infections are generally host specific with respect to infectivity and clinical symptoms…. The known human coronaviruses are notably fastidious in cell culture, preferring select cell lines, organ culture, or suckling mice for propagation. Coronaviruses grown in cell culture exhibit varying degrees of virulence and/or cytopathic effect (CPE) depending on the host cell type and culture conditions. Coronavirus have not previously been known to cause severe disease in humans but have been identified as a major cause of upper respiratory tract illness, including the common cold. Repeat infections in humans are common within and across serotype, suggesting that immune response to coronavirus infection in humans is either incomplete or short lived….The ability of animal-specific coronaviruses to cause severe disease raises the possibility that coronavirus could also cause more severe disease in humans….Past efforts to develop rapid diagnostics and vaccines for coronavirus infection in humans have been hampered by a lack of appropriate research models and the moderate course of disease in humans. Therefore, a need for rapid diagnostic tests and vaccines exists.”

Essentially from the above patent, the CDC patented the SARS-CoV virus and the PCR tests. What this means is that anyone without their express license could not verify the claim that the they had isolated the SARS CoV which they claimed was the causative agent of SARS. Hence, it may not be possible to verify that any therapy prescribed could be effective against SARS-CoV.

Notably, in 2003, the CDC claimed that the reason for seeking patents (of a virus- that is basically naturally occurring) is that to prevent others from monopolizing the field (https://apnews.com/article/145b4e8d156cddc93e996ae52dc24ec0)



Gain of function

In 2013, using funds from a NIH grant (Award Number: R01AI079231), Eco Health Alliance isolated a live virus from Chinese horseshoe bats that had high sequence identity to SARS-CoV and that can infect human cells using ACE2, the same receptor that is used by SARS-CoV (https://www.nature.com/articles/nature12711) .

From my understanding, they went hunting for viruses within bats that were easily transmissible to humans. They then made artificial viruses similar to SARS-Cov.

In a 2015 study (Engineered bat virus stirs debate over risky research - Lab-made coronavirus related to SARS can infect human cells: https://www.nature.com/articles/nature.2015.18787) , explored the above research and stated as follows “The researchers created a chimaeric (artificial) virus, made up of a surface protein of SHC014 and the backbone of a SARS virus that had been adapted to grow in mice and to mimic human disease. The chimaera infected human airway cells — proving that the surface protein of SHC014 has the necessary structure to bind to a key receptor on the cells and to infect them. It also caused disease in mice but did not kill them.”

However, the researchers, Ralph Baric, among others said that “the research did have benefits. The study findings “move this virus from a candidate emerging pathogen to a clear and present danger”, says Peter Daszak, who co-authored the 2013 paper. Daszak is president of the EcoHealth Alliance, an international network of scientists, headquartered in New York City, that samples viruses from animals and people in emerging-diseases hotspots across the globe”.

The Economics

However, discoveries of such viruses in of themselves do not create the kind of panic that drives more funding into further research and into vaccines development.

In a 2016, Peter Daszak is President of EcoHealth Alliance states the following with regard to how to make product development sustainable (products here being viruses, vaccines and other counter measures:

Lack of Public Understanding of Threats

Investors are interested at the height of a crisis, Daszak said. He pointed out that the share value for Roche Holding, a Swiss global health care company, increased during the H1N1 influenza pandemic. Unfortunately, as discussed, the interest and hype are short lived and focused around the outbreak. Daszak shared a story of a publication in Nature describing SARS in China and work done with colleagues from China's government-funded laboratory. The publication garnered no interest from the Chinese government, he said, and no one they talked with from the live animal markets seemed concerned about the findings. What was surprising for Daszak was how little interest was shown in the article from outside governments and the general public. Based on his experience and understanding, significant attention and interest should have come out of that article, but instead only a few virologists were interested in the paper for academic purposes—again showing the strong influence the media can have on public perception of threats.

Daszak also shared that during the recent Ebola outbreak, EcoHealth Alliance issued a press release and an analysis predicting which countries would be the first to be infected as a result of global air travel.2 The United States was predicted to be one of the top three countries that would receive infected individuals from countries with EVD, and it was predicted the patient would arrive into Dulles, Boston Logan, Newark, and/or JFK airport. They anticipated a lot of attention and coverage, but instead, again, there was very minimal pickup by the media. Daszak reiterated that, until an infectious disease crisis is very real, present, and at an emergency threshold, it is often largely ignored. To sustain the funding base beyond the crisis, he said, we need to increase public understanding of the need for MCMs such as a pan-influenza or pan-coronavirus vaccine. A key driver is the media, and the economics follow the hype. We need to use that hype to our advantage to get to the real issues. Investors will respond if they see profit at the end of process, Daszak stated
. (https://www.ncbi.nlm.nih.gov/books/NBK349040/)



Global Preparedness Monitoring Board

The board, in which Dr. Fauci is a member, Published the annual report “A world at Risk” in September 2019. The report indicated: A rapidly spreading pandemic due to a lethal respiratory pathogen (whether naturally emergent or accidentally or deliberately released) poses additional preparedness requirements. Donors and multilateral institutions must ensure adequate investment in development of innovative vaccine and therapeutics, surge manufacturing capacity, broad spectrum antivirals and appropriate non-pharmaceutical interventions. All countries must develop a system for immediately sharing sequences of any new pathogen for public health purposes, along with the means to share limited medical countermeasures across countries. (https://apps.who.int/gpmb/assets/annual_report/GPMB_Annual_Report_English.pdf)



Event 201

In October 2019, the John Hopkins Bloomberg School of Public Health together with the WEF and the bill and Melinda gates foundation presented the Event 201 Scenario. In hindsight, it essentially simulated the Covid 19 pandemic. (https://www.centerforhealthsecurity.org/event201/scenario.html )



Covid first case

First reported on 31 Dec 2019 when
Wuhan Municipal Health Commission, China, reported a cluster of cases of pneumonia in Wuhan, Hubei Province. A novel coronavirus was eventually identified (https://www.who.int/news/item/27-04-2020-who-timeline---covid-19 )



Alternative Therapies

A 2005 research noted that chloroquine could inhibit infection and spread of SARS CoV at clinically admissible concentrations ( https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1232869/ ).

A summary of the study

Results

We report, however, that chloroquine has strong antiviral effects on SARS-CoV infection of primate cells. These inhibitory effects are observed when the cells are treated with the drug either before or after exposure to the virus, suggesting both prophylactic and therapeutic advantage. In addition to the well-known functions of chloroquine such as elevations of endosomal pH, the drug appears to interfere with terminal glycosylation of the cellular receptor, angiotensin-converting enzyme 2. This may negatively influence the virus-receptor binding and abrogate the infection, with further ramifications by the elevation of vesicular pH, resulting in the inhibition of infection and spread of SARS CoV at clinically admissible concentrations.

Conclusion

Chloroquine is effective in preventing the spread of SARS CoV in cell culture. Favorable inhibition of virus spread was observed when the cells were either treated with chloroquine prior to or after SARS CoV infection. In addition, the indirect immunofluorescence assay described herein represents a simple and rapid method for screening SARS-CoV antiviral compounds.


However, this has been ignored as a possible therapy.

My conclusion

More info and personal research is needed. The pandemic seems to have much economic value to the researchers, the drug companies producing the vaccines. Its been a long time coming for Corona viruses’ researchers (Ralph Baric, Dr. Fauci etc). As it appears, it will be a very sustainable product, with possible global immunization and subsequent “booster” shots with time. It comes at an opportune time when HIV/AIDS has been on the decline with major donors such as USAID scaling down funding for this.

Primary Source Document: https://www.davidmartin.world/wp-content/uploads/2021/01/The_Fauci_COVID-19_Dossier.pdf
 

Riva

Lister
It’s good to always dig deeper…to seek for better understanding…like this study that shows where the vaxxine goes in your body (which organs) upon injection - see page 6 and 7

i.e Adrenal glands, bone marrow, ovaries, and spleen being the major ones.

The bold one makes me curious about research on menstrual cycle pré and post vaccination.

 
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Ngimanene na Muchere

Elder Lister
It’s good to always dig deeper…to seek for better understanding…like this study that shows where the vaxxine goes in your body (which organs) upon injection - see page 6 and 7

i.e Adrenal glands, bone marrow, ovaries, and spleen being the major ones.

The bold one makes me curious about research on menstrual cycle pré and post vaccination.

Oh well, when I say women are being sterilized.....
 
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